VistaPlex™ Multiplexing Assay Kits Powered by EpicIF Technology

Accelerate spatial proteomics with biologically relevant, pre-validated assay kits designed for the CellScape™ platform. VistaPlex Assay Kits offer a robust starting point for any spatial proteomic study and are easily customizable using EpicIF™ technology, giving you both reproducibility and flexibility in one streamlined workflow.

VistaPlex Spatial Immune Profiling Kit

Explore Available VistaPlex Assay Kits

Human FFPE VistaPlex Kits

Human FFPE Spatial Immune Profiling Assay Kit

Human FFPE Tissue Architecture Kit

Human FFPE Cell Boundaries Kit

Coming Soon: Human FF Spatial Immune Profiling Kit

Mouse VistaPlex Kits

Coming Soon: Mouse FFPE Spatial Immune Profiling Kit

Coming Soon: Mouse FF Spatial Immune Profiling Kit

Panels That Flex with Your Science

Modular

Modular

Expand your assay and scale your plex without revalidating or redesigning your workflow.

Validated for Publication-Quality Spatial Proteomics

Rigorous Antibody Selection

Rigorous Antibody Selection

Every clone is screened for specificity, sensitivity, and reproducibility using stringent criteria across tissue types:

  • Must meet minimum qualitative specificity scores
  • Required signal-to-noise ratio of ≥ 2
  • Consistent performance across serial tissue sections
  • Suitable for a wide set of tissue types

Antibody Conjugate Validation

Antibody Conjugate Validation

Each antibody-dye conjugate is verified for correct expression, localization, and target specificity:

  • Evaluated with orthogonal counterstains
  • Localization confirmed in immune and tumor compartments
  • No off-target or artifactual background signal allowed
  • Staining patterns must match known cellular biology

Multiplex Optimization

Multiplex Optimization

We ensure multiplex panels perform as reliably as singleplex—while minimizing imaging cycles:

  • Each antibody tested in singleplex and full multiplex panel
  • Retains distinct, biologically appropriate staining in multiplex
  • No signal bleed, steric hindrance, or cross-reactivity
  • Multiplex mirrors singleplex performance localization and intensity

Robust Reproducibility

Robust Reproducibility

Our validation process includes multiple sample types and rigorous reproducibility testing:

  • Validated on five tissue types with ≥ 3 replicates per type
  • All antibodies meet validation criteria across tissues
  • Most markers show <25% coefficient of variation
  • Cross-operator reproducibility confirmed through independent runs
Antibody_Validation_Lab_Image

Store, Seal, and Revisit Later

With the CellScape Whole‑Slide Imaging Chamber and VistaPlex Assay Kits, your samples go beyond a one-and‑done experiment. Seal slides after imaging to preserve tissue integrity and revisit samples days or weeks later. This flexible workflow supports iterative assay expansion without needing to prepare a new tissue sample, enabling data-driven decisions and efficient use of precious samples.

Ready to Accelerate Your CellScape Journey?

Jump start your next spatial biology project with the efficient and reliable antibody panels and protocols of VistaPlex Multiplex Assay Kits.

Related Products

CellScape instrument

CellScape Instrument

Bring the power of CellScape to your lab and experience high-resolution imaging, walkaway automation, versatile detection assays, and high multiplex capability.

Leverage-our-CellScape-Services

Leverage our CellScape Services

We offer spatial biology services for labs who do not yet have their own instrumentation. The VistaPlex Assay Kits can be used to expedite your CellScape service project.

Resources

Tech Note

VistaPlex Assay Kit Design and Validation Process

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Tech Note

Data-Driven Expansion: A Modular Approach to Quantitative Spatial Phenotyping

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Webinar

Building High-Plex Spatial Proteomic Panels — A Simplified, Flexible Workflow Using the CellScape Platform

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