VistaPlex™ Cell Boundaries Assay Kit
Enabling Precise Single-Cell Segmentation using the CellScape™ Platform
Achieve clean cell boundary identification to support cell segmentation in human FFPE samples using the Cell Boundaries Assay Kit.
VISTAPLEX-3101-10RXN
Precise Spatial Proteomics Starts Here
The Cell Boundary Assay Kit delivers fluorescently labeled antibodies for cell boundary staining optimized by our robust validation workflow, enabling clear delineation of individual single cells within intact tissue architecture. By improving segmentation accuracy, the kit ensures each protein signal is correctly assigned to its cell of origin, resulting in cleaner, more reliable data for cell type classification, spatial mapping, and neighborhood analysis. The panel can be expanded by pairing with additional VistaPlex assay kits or your markers of choice.
Segment your cells with confidence
| Marker | |
|---|---|
| Cell Localization | |
| STYOX Orange | Nucleus |
| ATP1A1 | Surface Membrane |
| Lamin B1 | Nuclear Envelope |
| B2M | Surface Membrane |
Convenient and Efficient Spatial Multiplexing
The VistaPlex Cell Boundaries Assay Kit enables automated staining and imaging of 10 human FFPE samples for 4 cell segmentation biomarkers using the CellScape Precise Spatial Proteomics platform.
Validated Antibodies
The kit includes 4 validated, fluorescently labeled biomarkers to support cell segmentation.
Reagents and Buffers
Antibody diluent is provided for preparing antibody cocktails; EpicIF Buffer and Wash Buffer are available separately.
Optimized Protocol
An online protocol is available to guide consistent and reliable execution of the multiplexed assay.
Validation Ensures Reliable Antibody Panel Performance
Antibodies for the multiplex panel were validated using eight human tonsil FFPE tissue samples to ensure specificity, sensitivity, and reproducibility. Additional FFPE tissue types were validated for broad sample suitability. Download the validation report for experiment details and results.
Data Summary
| FFPE Tissue Type | |
|---|---|
| Suitability | |
| Tonsil |
|
| Breast Cancer |
|
| Melanoma |
|
| Colon Cancer |
|
| Head and Neck Cancer |
|
Ready to Accelerate your CellScape Research?
Expedite your immunophenotyping with the efficient and reliable VistaPlex Cell Boundaries Assay Kit: VISTAPLEX-3101-10RXN.
Need Higher Plex?
Expand the Cell Boundaries Kit with modular VistaPlex Assay Kits to build a customized Precise Spatial Proteomics panel. Compatible kits can be run on the same sample, and the CellScape workflow lets you seal, store, and revisit the same slide. Add new biomarkers later to drive data-driven panel expansion and make the most of precious samples.
Explore our Assay Solutions
Browse More VistaPlex Assay Kits
Learn more about how our catalog of VistaPlex Assay kits can expedite development and accelerate precise data generation for CellScape multiplexed assays.
Leverage our CellScape Services
We offer spatial biology services for labs who do not yet have their own instrumentation. The VistaPlex Assay Kits can be used to expedite your CellScape service project.
Additional CellScape Products Needed
Whole Slide Imaging Chambers, product PRSM-CS-WSIC, are required for converting slides into microfluidic chambers required for staining with the VistaPlex Assay Kits. Wash Buffer, product PRSM-BUF-WASH, is required for washing samples in between staining cycles. EpicIF Buffer, product PRSM-BUF-EPIC, is required for signal removal between cycles.
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